Concentration-response data were collected in multiple β2AR-assays. Pharmacological modeling enabled a quantification of receptor activation per ligand. This way,  pair-wise comparison of signaling pathways was reduced to a difference between so-called transduction  ratios (ΔΔlog(τ/KA)), which were tested for significant  difference to a reference. Analyses per ligand were summed-up as fingerprints in a heat-map matrix.